Assessment of histopathological effect of factory effluents on the liver and kidney tissues of chickens (Gallus gallus domestica) reared around factory sites in Nnewi Metropolis, Anambra State, Nigeria

A N Okpogba, E C Ogbodo, M N Izuogu, A K Amah, D C Ejiofor, I Wopara, F N Ujowundu, C Nwanegwo, E U Modo, C N Chinaka


Exposure to factory effluents comes with deleterious consequences. The purpose of this study was to assess the histopathological effect of factory effluents on the liver and kidney tissues of chickens (Gallus gallus domestica) reared around factory sites in Nnewi Metropolis, Anambra State, Nigeria. A total of twenty-nine chicks comprising of sixteen chicks exposed to factory sites and thirteen non-exposed chicks were grown to adult birds (chickens) for the study and were allowed to feed from the surrounding homeland of the factories including lead acid battery manufacturing factory (A), metal fabricating factory (B) and metal forging factory (C) respectively until they were aged between four and five months as adult birds (chickens) for the study. The chickens were sacrificed to obtain the liver and kidney tissues for histological analyses. The histopathological evaluation of the effect of the factory effluents on the liver and kidney tissues of birds reared around the four factories (A-D) and control were done by microscopic examination of haematoxylin and eosin (H&E) stained sections. The results obtained in factory A, B and C showed widening of the liver central vein and thickening of the vessels with invasion of the surrounding areas by chronic inflammatory cells as against the controls which showed fairly normal liver tissues with normal liver parenchyma cells with a central vein containing blood cells. Also, the photomicrographs of the kidney tissues of birds reared around factory A, B and C showed evidence of marked glomerulo-nephritis and hypercellularity with evidence of glomerulosclerosis and hyalinization of the glomeruli as against the normal glomeruli and tubules found in the control birds. These results indicate that the environment where these birds are reared is a potential threat to the lives of the birds reared around these factories.

Keywords: Factory site, Factory effluents, Liver, Kidney, Chicken (Gallus gallus domestica), Nnewi metropolis.

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Barbara Y, Geraldine O, Phillip T. Histology of the digestive tract: Wheater′s Functional Histology. 2014; 11th ed. Philadelphia: Elsevier, Churchill Living Stone, Pp. 274-80.

Singh I. Digestive System: Textbook of Human Histology. New Delhi, India: Jaypee Brother′s Medical Publishers (P) Lt. 2014;249-57.

Guyton AC, Hall JE. Metabolism and temperature control: The liver as an organ. In: Hall JE, editor. Guyton and Hall Textbook of Medical Physiology. 2010; Vol. 13. 12th ed. Philadelphia, Pennsylvania: Saunders, Pp. 536-40.

Sáez L, Zuviæ T, Amthauer R, Rodríguez E, Krauskopf M. Fish liver protein synthesis during cold acclimatization: Seasonal changes of the ultrastructure of the carp hepatocyte. Journal of Experimental Zoology 2012;230:175-86.

Emmanuel IO, Emmanuel IO. Comparative histologic anatomy of vertebrate liver. Ann Bioanthropol 2015;3(1):1-5.

Tim, N. What do the kidneys do?. Medical News Today 2019;1:1-5.

Al-Ajeely, RA, Fadhil SM. Morphohistological study on the development of kidney and ureter in hatching and adulthood racing pigeon (columba livia). Domestica J 2012;3(3):665-77.

Casotti, G., Lindberg KK, Braun EJ. Functional morphology of the avian medullar cone. Am J Physiol, Regul, Integr Comp Physiol 2000;279:1722-5.

Reece WO. Dukes’ Physiology of Domestic Animals. 2004; 12th Ed. Cornell University Press, Ithaca, Pp. 107-113.

Kaplan O, Yildirim NC, Yildirim N, Cimen N. Toxic Elements in animal products and environmental health. Asian J Anim Vet Advancement 2011;6:228-32.

Rajaganapathy, V. Effect of pollution in livestock production systems. Proceedings of the National Seminar on Recent Trends in Animal Welfare and Production 2006;1-3.

Rajaganapathy V, Xavier F, Sreekumar D, Mandal PK. Heavy metal contamination in soil, water and fodder and their presence in livestock and products: a review. J Environ Sci Technol 2011;4(3):234-49.

Gingerich WH, Weber LJ. Hepatic Toxicology of Fishes. In: Aquatic Toxicology, Weber, L.J. (Ed.). Raven Press, New York, 1982:55-105.

Montaser, M., Mahfouz, M. E., El-Shazly, S. A. M., Abdel-Rahman, G. H. and Bakry, S. Toxicity of heavy metals on fish at Jeddah Coast KSA: Metallothionein expression as a biomarker and histopathological study on liver and gills. World J Fishes Mar Sci 2010;2:174-85.

Abdel-Baki, A. S., Dkhil, M. A. and Al-Quraishy, S. Bioaccumulation of some heavy metals in tilapia fish relevant to their concentration in water and sediment of Wadi Hanifah. Saudi Arabian. Afri J Biotechnol 2011;10:2541-7.

Miyase, C. A., Arzu, Y., Ilkay, Y., Ertan, O., Ozkan, D. and Metin, Ar. Cadmium Induced Changes on Growth Performance, Some Biochemical Parameters and Tissue in Broilers: Effects of Vitamin C and Vitamin E. Asian J Anim Vet Adv 2011;6(9:923-34.

Luna, L.G. Manual of Histopathologic Staining Methods of the Armed Forces Institute of Pathology. 1968, 3rd edn. McGrew-Hill Book Company, London.

Okpogba, A.N., Ogbodo, E.C., Ugwu, E.C., Oguaka, V.N., Dike, C.C., Ujowundu, F.N. Comparative assessment of heavy metal levels in chickens (Gallus gallus domestica) in rural (Elele) and urban (Nnewi) Areas. Asian J Sci Technol 2018;9(11):9056-9.

Almansour, M.I. Inter-Species differences between lead concentration in the feathers of Bayer, W.N., Spann, J.W., Sileo, L. and Franson, J.C. Lead poisoning in six captive avaian species. Arch Environ Contamination Toxicol 1988;17:121-30.

Pycnonotus leucogenys and Streptopelia senegalensis from different cities of Saudi Arabia. Int J Zoological Res 2007;3:200-6.

John, H.H and Jeanne, I.R. Food additives, contaminants and natural toxins In: Maurice E.S., James, A.O., Moshe, S.L. and Febiger, (eds.) Modern nutrition in health and disease, 1994; 8th ed., part II. Pp: 15]